Data acquisition and processing for UCNPs fluorescence lifetime measurement
نویسندگان
چکیده
منابع مشابه
Fluorescence lifetime imaging by using time-gated data acquisition.
The use of the time gating technique for lifetime reconstruction in the Fourier domain is a novel technique. Time gating provides sufficient data points in the time domain for reliable application of the Fourier transform, which is essential for the time deconvolution of the system of the integral equations employed in the reconstruction. The Fourier domain telegraph equation is employed to mod...
متن کاملblending and deblending in seismic data acquisition and processing
in the current seismic data acquisition techniques, sources are fired with large time intervals in order to avoid interferences between the responses of successively firing sources, measured by the receivers. this leads to a time-consuming and expensive survey. theoretically the waiting time between two successively firing sources has to be infinite, since the wavefield never vanishes completel...
متن کاملTime-domain measurement of fluorescence lifetime variation with pH
Advances in the design and miniaturization of the lasers and electronics required for Time Correlated Single Photon Counting (TCSPC) measurement of fluorescence lifetime have simplified the use of the time domain method. We have assembled a compact portable system that is capable of measuring lifetimes down to ~200 ps (with deconvolution) and that can operate at a range of excitation and emissi...
متن کاملNear-Field Fluorescence Imaging and Fluorescence Lifetime Measurement of Light Harvesting Complexes in Intact Photosynthetic Membranes
Fluorescence lifetime measurements with nanometer spatial resolution are made possible by incorporating time-correlated single photon counting with a near-field fluorescence microscope. Using this apparatus, single pieces of intact photosynthetic membrane from the Chlamydomonas reinhardtii strain doubly deficient in photosystem I (PS I) and photosystem I1 (PS 11) are examined. Comparison of sim...
متن کاملFluorescence lifetime standards for time and frequency domain fluorescence spectroscopy.
A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 degrees C were measured independently by nine laboratories using single-photon timing and multifrequency phase and modulation fluorometry instruments with lasers as excitation source. The dyes that can serve as fluorescence lifetime standards for time-domain and frequency-domain measurements are all co...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Applied Optics
سال: 2019
ISSN: 1002-2082
DOI: 10.5768/jao201940.0401006